Researchers develop synthetic scaffolds to heal injured tendons and ligaments. The researchers are the first to develop and patent novel fibre-reinforced hydrogel scaffolds, a synthetic substance that has the ability to mimic and replace human tendon and ligament tissue. : science
Related Article: https://www.eurekalert.org/pub_releases/2020-04/uos-rds041420.php
High-strength fiber-reinforced composite hydrogel scaffolds as biosynthetic tendon graft materials
– Young Jung No, Solaiman Tarafder, Barbara Reischl, Yogambha Ramaswamy, Colin R. Dunstan, Oliver Friedrich, Chang Lee, and Hala Zreiqat
The growth of appropriate synthetic scaffolds to be used as human tendon grafts to restore tendon ruptures stays a important engineering problem. Previous synthetic tendon grafts have demonstrated suboptimal tissue ingrowth and synovitis due to put on particles from fiber-to-fiber abrasion. In this examine, we current a novel fiber-reinforced hydrogel (FRH) that mimics the hierarchical construction of the native human tendon for synthetic tendon graft materials. Ultrahigh molecular weight polyethylene (UHMWPE) fibers had been impregnated with both biosynthetic polyvinyl alcohol/gelatin hydrogel (FRH-PG) or with polyvinyl alcohol/gelatin + strontium-hardystonite (Sr-Ca2ZnSi2O7, Sr-HT) composite hydrogel (FRH-PGS). The scaffolds had been fabricated and assessed to consider their suitability for tendon graft functions. The microstructure of each FRH-PG and FRH-PGS confirmed profitable impregnation of the hydrogel part, and the tendon scaffolds exhibited equilibrium water content material of ∼70 wt %, comparable to the values reported for native human tendon, in contrast to ∼50 wt % water content material retained in unmodified UHMWPE fibers. The tensile power of FRH-PG and FRH-PGS (77.zero–81.eight MPa) matched the vary of human Achilles’ tendon tensile strengths reported in the literature. In vitro tradition of rat tendon stem cells confirmed cell and tissue infiltration into each FRH-PG and FRH-PGS after 2 weeks, and the presence of Sr-HT ceramic particles influenced the expression of tenogenic markers. On the different hand, FRH-PG supported the proliferation of murine C2C12 myoblasts, whereas FRH-PGS seemingly didn’t help it underneath static tradition circumstances. In vivo implantation of FRH-PG and FRH-PGS scaffolds into full-thickness rat patellar tendon defects confirmed good collagenous tissue ingrowth into these scaffolds after 6 weeks. This examine demonstrates the potential viability for our FRH-PG and FRH-PGS scaffolds to be used for off-the-shelf biosynthetic tendon graft materials.